Lipid Analysis by Quantitative Thin-layer Chromatography.

Quantitative Two-dimensional Thin-layer Chromatography of Naturally Occurring Phospholipids.

Gradient-thickness thin-layer chromatography for the isolation and analysis of trace amounts of free fatty acids in large lipid samples.

A thin-layer chromatographic method for quantitative isolation of free fatty acids is described. This method appears to be more satisfactory than existing methods in offering the combination of advantages of specificity, simplicity, rapidity, reproducibility, accuracy, high sensitivity, and applicability as a preparative technique. The method involves chromatography on a thin-layer plate on whi...

Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones.

A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas-liquid chromatography. Identification of the plasmalogen...

Separation of phosphatidylinositols and other phospholipids by two-step one-dimensional thin-layer chromatography.

A quick and efficient thin-layer chromatographic procedure is described for the separation of phosphatidylinositol-4,5-bisphosphate, phosphatidylinositol-4-monophosphate, phosphatidylinositol, phosphatidylcholine, phosphatidic acid, and phosphatidylethanolamine. The method involves development of the phospholipids successively in two different solvent systems but in the same direction. The meth...

Application of thin-layer chromatography to the quantitation of plasma neutral lipids and free fatty acids.

THIN-LAYER CHROMATOGRAPHY (TLC) has been widely applied to the qualitative analysis of complex biologic lipid mixtures (1, 2), as well as to the quantitation of selected biochemical entities (3). Quantitation by TLC has been limited,for the most part,to the recovery of separated components from the chromatoplate, followed by an independent analysis using established analytic methods. Current te...

Rapid quantitative measurement of lung tissue phospholipids.

A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmission densitometry.